FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n01_HBLm29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n01_HBLm29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32872654
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2395240.7286421108560325TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG302850.043.15969544-45
CGTATGC309150.042.5888346-47
TATGCCG312250.042.0287248-49
CTCGTAT294800.041.712144-45
TCTCGTA308750.038.3597942-43
GTCACGA354000.037.55727828-29
ATGCCGT364600.035.89646548-49
CGAGATT373850.035.5671432-33
TCACGAG377500.035.4753930-31
GTATGCC373300.035.2383246-47
AGTCACG384450.034.84200328-29
CCGTCTT376200.034.7012552-53
ATCTCGT343950.034.2613842-43
GCCGTCT382150.034.1607550-51
CACGAGA394700.033.7609930-31
ACGAGAT400400.033.33923732-33
GAGCACA816650.033.3313379
TGCCGTC395650.033.23520350-51
AGAGCAC833200.032.4982878
CGGAAGA877150.030.7326934
Produced by FastQC (version 0.11.5)