FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l02n02_brain_scrnaseq_4.35200000098fac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l02n02_brain_scrnaseq_4.35200000098fac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33232903
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC781940.23529091033666244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1137650.074.224391
AGCAGTG1154500.072.7039642
GGTATCA1292500.064.9626858
GCAGTGG1315750.063.8396953
GTATCAA1333600.063.390279
CAGTGGT1353850.062.1322674
TGGTATC1391800.060.4568827
GTGGTAT1395650.060.316456
AGTGGTA1412800.059.585765
AGAGTAC1217100.036.5964318-19
GCAGAGT1277400.034.9320716-17
CAACGCA1290800.034.8416712-13
TACATGG1261300.034.82700322-23
CATGGGG847350.034.6786524-25
CAGAGTA1242100.033.53859318-19
ATCAACG1372200.033.02289610-11
GAGTACA1287650.032.34399420-21
AGTACAT1399000.031.88195220-21
ACATGGG1292800.031.67576824-25
AACGCAG1353400.031.01673114-15
Produced by FastQC (version 0.11.2)