FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l02n02_brain_scrnaseq_3.35200000098f82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l02n02_brain_scrnaseq_3.35200000098f82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35324025
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC915860.2592739643910908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1327300.076.970151
AGCAGTG1393600.072.919552
GGTATCA1534750.066.051588
GCAGTGG1551100.065.428463
CAGTGGT1598500.063.4999354
GTATCAA1613550.063.1860439
TGGTATC1610550.063.0332837
GTGGTAT1646100.061.739226
AGTGGTA1660000.061.2228745
AGAGTAC1478350.036.4300918-19
GCAGAGT1516250.035.54802716-17
CAACGCA1543550.035.2616312-13
CATGGGG1014200.034.72677624-25
TACATGG1525700.034.63607422-23
CAGAGTA1514150.033.30584718-19
ATCAACG1670700.032.79347210-11
AACGCAG1563900.032.42064314-15
GAGTACA1564950.032.22812320-21
AGTACAT1688150.031.90436620-21
ACGCAGA1708950.031.83543614-15
Produced by FastQC (version 0.11.2)