FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l02n02_brain_scrnaseq_2.35200000098f68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l02n02_brain_scrnaseq_2.35200000098f68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35142089
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC871630.24803021812391401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1298100.076.6720051
AGCAGTG1359350.072.8336262
GGTATCA1501050.065.991468
GCAGTGG1519400.065.2898253
CAGTGGT1558250.063.7871444
GTATCAA1575850.063.260189
TGGTATC1577450.062.9281967
GTGGTAT1610950.061.6510246
AGTGGTA1624500.061.2429435
AGAGTAC1441700.036.4388518-19
GCAGAGT1483950.035.52298716-17
CAACGCA1513600.035.1757212-13
CATGGGG992850.034.79263324-25
TACATGG1497400.034.63844722-23
CAGAGTA1472600.033.38834818-19
ATCAACG1626200.032.9103610-11
AACGCAG1540800.032.1857814-15
GAGTACA1527100.032.1486320-21
AGTACAT1653950.031.7883620-21
ACATGGG1522750.031.68760924-25
Produced by FastQC (version 0.11.2)