FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l02n02_brain_scrnaseq_1.35200000098f5c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l02n02_brain_scrnaseq_1.35200000098f5c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28564638
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC619920.21702357999425723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT975950.074.906721
AGCAGTG1006350.072.296212
GGTATCA1126350.064.544748
GCAGTGG1147700.063.465083
GTATCAA1160100.062.9059339
CAGTGGT1180450.061.7515264
TGGTATC1213500.060.0687187
GTGGTAT1215550.059.9592446
AGTGGTA1229300.059.3731775
AGAGTAC1061650.036.48933818-19
GCAGAGT1112200.034.8533616-17
TACATGG1099050.034.7281122-23
CAACGCA1126450.034.71592312-13
CATGGGG724450.034.693424-25
CAGAGTA1088300.033.12715518-19
ATCAACG1193600.032.9335710-11
GAGTACA1124450.032.14130820-21
AGTACAT1217800.031.8854720-21
ACATGGG1125200.031.53269824-25
AACGCAG1167850.031.05297514-15
Produced by FastQC (version 0.11.2)