FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l02n01_brain_scrnaseq_4.35100000098faf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l02n01_brain_scrnaseq_4.35100000098faf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33232903
Sequences flagged as poor quality0
Sequence length101
%GC25

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC88450.078.75211
CCTTACG88350.077.227841
AGACTAG73300.075.749028
GACTAAC63800.074.5485841
GAACCTA129750.074.118931
GTACTCC95000.073.622431
TGACTAG66450.073.291711
CTAAGAC89750.073.111421
GCTGCAG90700.072.764731
GCATGCG96800.072.498851
CTAGCCT102000.072.203381
GTGCAGC96800.072.057081
GACACGC125550.071.9813161
CCTACCA81650.071.926391
GGGACCT90500.071.245471
CTCGGGA98550.071.115021
GTAACTG91300.070.4650651
TACGGGC96000.070.1333541
AGGCTAG114900.069.819418
ATGCGAT110200.069.3745961
Produced by FastQC (version 0.11.2)