FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l02n01_brain_scrnaseq_3.35100000098f85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l02n01_brain_scrnaseq_3.35100000098f85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35324025
Sequences flagged as poor quality0
Sequence length101
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC97400.078.005361
CCTTACG98950.077.935921
GCATGCG118550.076.794281
AGACTAG87250.076.049698
GAACCTA156250.074.717111
TGACTAG80400.073.282551
GTACTCC115450.072.970911
CTAGCCT125450.072.911331
GACTAAC80900.072.829631
GTAACTG110500.072.58471
CTAAGAC106050.071.866851
GACACGC145300.071.747361
GCTGCAG113450.071.744361
GTGCAGC111850.071.623661
AGGCTAG136900.071.540458
ATGCGAT136250.070.689061
GATCGCG85050.070.6726151
CCTACCA100150.070.597111
TACGGGC111350.070.025031
CTCGGGA121900.069.421691
Produced by FastQC (version 0.11.2)