FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l02n01_brain_scrnaseq_2.35100000098f6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l02n01_brain_scrnaseq_2.35100000098f6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35142089
Sequences flagged as poor quality0
Sequence length101
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG105900.078.6539151
CGCTATC102650.078.320561
AGACTAG92200.076.448668
GCATGCG123200.074.551361
GAACCTA155250.074.18831
GTACTCC114550.073.4611741
GACTAAC80300.073.432891
CTAGCCT117950.073.196681
TGACTAG78950.072.762661
CTAAGAC106150.072.336211
GTAACTG108000.072.109021
GCTGCAG111500.072.104091
CCTACCA96850.071.775981
GATCGCG81600.071.564251
GACACGC149050.071.536111
GGGACCT104000.071.227421
GTGCAGC113350.071.136861
AGGCTAG127650.070.994468
ATGCGAT132250.070.886881
TACGGGC107100.070.1417851
Produced by FastQC (version 0.11.2)