FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l02n01_brain_scrnaseq_1.35100000098f5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l02n01_brain_scrnaseq_1.35100000098f5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28564638
Sequences flagged as poor quality0
Sequence length101
%GC24

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC78300.081.5588761
CCTTACG79200.077.752351
GCATGCG90350.077.4128951
AGACTAG63000.076.297078
GAACCTA111850.074.8095551
GTACTCC85850.074.773681
CTAGCCT88250.073.978531
CTAAGAC79650.073.8530651
CTCGGGA85000.073.8444061
GACACGC108650.073.2518841
GGGACCT80350.071.908681
GTGCAGC83200.071.786971
GACTAAC56500.071.3991241
GTAACTG76750.071.381331
GCTGCAG83250.071.230181
AGGCTAG101900.070.336868
TGACTAG57100.070.14961
GATCGCG59350.070.052081
GGCTCGA50000.068.707151
ATGCGAT101800.068.23911
Produced by FastQC (version 0.11.2)