FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l01n02_sbc_index.35200000098f4f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l01n02_sbc_index.35200000098f4f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32891218
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAACCACGAAAGAATT585836317.81132884771856No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAGGTGCGTGTGAATT388305611.805753134468903No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAGGTGGTACGGAATT32101659.759945648713892No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAAGGCAGCTGGAATT29302738.908982938850121No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAACCAAACGCAGAATT20881366.348612568862606No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAGCGGTCCGTGAATT17357935.277375255607743No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAACCTAACCACGAATT15109764.593858457902045No Hit
GACCTCCCTAGCAAACTGGGGCACAAACTTAATTAAGAATTCCAGCTGAG10855973.3005679509953083No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAGGTGCGCCGTCACA10602183.2234075369297663No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAACCCGTGTTAGAATT10389303.158685093388758No Hit
GACCTCCCTAGCAAACTGGGGCACGCTGTTTGGTCTGCTTTCTGACAAAC4581011.3927760291516111No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAGCTAAGGTTATGTA4179581.270728253359301No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAACCACGAAAAAAAA2142640.6514322455313147No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAGCGGTCCGTGCTTC2067520.6285933223877571No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAGGTGGTACGGAATG1288970.3918888014423789No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAACCACGAAAGTTAT1158250.35214567000832864No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTACAAGAGATTAGCGTGGCTG882370.2682691775050714No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAGGTGTACGGGCAGA772900.23498673718923999No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAAAATCGGGAAAAACGGGAA720970.21919832825892915No Hit
GACCTCCCTAGCAAACTGGGGCACGAGCAGATTGTACTGAGAGTGCACCA635240.19313362004411025No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAACCCGTGGGGCACAA625150.19006593188491835No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAGTGCTTTCAGTGGG610880.18572738777870737No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAACCACGAAAGAAAG597300.18159862611351152No Hit
GACCTCCCTAGCAAACTGGGGCACAAACTTAACTCTGCTGAAGCCAGTTA528340.1606325433129293No Hit
GACCTCCCTAGCAAACTGGGGCACACGCGGCAACGCTATTCGACTGGTAT463060.140785300197761No Hit
GACCTCCCTAGCAAACTGGGCACAAGCTTAATTAAACCACGAAAGAATTC441080.13410266533759863No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAGCGGTCCGTGTTTA432550.13150926791461479No Hit
GACCACCCTAGCAAACTGGGGCACAAGCTTAATTAAACCACGAAAGAATT406390.12355577710743335No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAAGGCAGCTCTTATA403770.12275921189662238No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAACCCGTGTTTTTAGT399990.12160996895888745No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAAGTAGGCTCCCATG374090.11373552660774071No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAGGTGGTACTGACAG369810.11243426740840062No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAAGGCAGTGTATACT340440.10350483220171415No Hit
GACCTCCCTAGCAAACTGGGGCACAAGCTTAATTAAACCACTGGGGCACA331430.10076549916758935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTCC30738050.093.834361
ACCTCCC30712850.093.428082
CCTCCCT30687450.093.2649463
TCCCTAG30691250.092.9013065
CTCCCTA30766400.092.847864
CCCTAGC30939650.092.618956
ACCTCCT161550.092.430392
CCTAGCA31138150.092.1440057
GACCTCT58200.092.108951
CTAGCAA31189900.092.001348
TAGCAAA31131650.091.935859
CACCCTA220800.091.7572564
GACTCCC68800.091.4953461
CCTCCTA131750.091.246663
GACCTTC34050.091.126161
TGCCTAG19950.091.059675
GACCTAG24850.091.049871
CCACCCT222900.090.808543
CCTCTAG31550.090.163613
GACCTGC21950.090.033941
Produced by FastQC (version 0.11.2)