FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l01n02_perturseq_pilot_run_4.35200000098fd2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l01n02_perturseq_pilot_run_4.35200000098fd2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20285915
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC220320.10860737610307448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA268150.052.4535148
AAGCAGT280550.050.671121
TGGTATC282600.050.0206347
GTGGTAT297950.047.490916
GTATCAA300500.046.962929
GCAGTGG320150.044.3444143
AGCAGTG328950.043.1581152
CAGTGGT335100.042.1839184
AGTGGTA352500.040.20825
AGAGTAC245000.031.08156418-19
GCAGAGT256100.029.72491616-17
CAACGCA272050.028.2667412-13
TACATGG276400.027.30584722-23
CAGAGTA263150.026.75230218-19
AACGCAG267050.026.43154314-15
CATGGGG197150.025.8042324-25
TCAACGC310800.022.86943812-13
GAGTACA319650.022.2884920-21
CTTAGAG53850.021.15760286-87
ACATGGG338250.020.5438924-25
Produced by FastQC (version 0.11.2)