FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l01n02_perturseq_pilot_run_3.35200000098fb8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l01n02_perturseq_pilot_run_3.35200000098fb8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19856407
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC224170.11289555053943041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA271550.052.6449748
AAGCAGT279550.051.7432631
TGGTATC285100.050.1923077
GTGGTAT300050.047.675836
GTATCAA302300.047.522889
GCAGTGG323150.044.529293
AGCAGTG334950.043.0586782
CAGTGGT335650.042.8709684
AGTGGTA354150.040.6314855
AGAGTAC246700.031.27746218-19
GCAGAGT257800.029.93945116-17
CAACGCA274800.028.3688412-13
TACATGG281850.027.23695222-23
CATGGGG196050.026.85976224-25
CAGAGTA266600.026.60060718-19
AACGCAG269200.026.5611614-15
TCAACGC312700.023.00869812-13
GAGTACA315600.022.68684820-21
CTTAGAG53300.021.86140886-87
ACATGGG336400.021.19476324-25
Produced by FastQC (version 0.11.2)