FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l01n02_perturseq_pilot_run_2.35200000098f9f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l01n02_perturseq_pilot_run_2.35200000098f9f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30433182
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC349370.11479903744537788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA417300.052.784938
AAGCAGT432650.051.5416871
TGGTATC442050.049.967617
GTGGTAT463600.047.7866826
GTATCAA466000.047.5405739
GCAGTGG497300.044.6522183
AGCAGTG515000.043.245192
CAGTGGT517400.043.0445944
AGTGGTA543300.040.8975335
AGAGTAC382650.031.63565618-19
GCAGAGT401550.030.29260816-17
CAACGCA425650.028.78101312-13
TACATGG438200.027.43395422-23
CAGAGTA411650.026.78960418-19
AACGCAG418900.026.57776314-15
CATGGGG312150.026.48784624-25
TCAACGC487700.022.90521412-13
GAGTACA496950.022.38533620-21
CTTAGAG84650.020.92445486-87
ATCAACG600700.020.75342210-11
Produced by FastQC (version 0.11.2)