FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l01n02_perturseq_pilot_run_1.35200000098f75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l01n02_perturseq_pilot_run_1.35200000098f75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17682127
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC190120.10752100129130392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA231150.052.1360138
AAGCAGT241900.050.4208761
TGGTATC243650.049.4612777
GTGGTAT258650.046.68366
GTATCAA265150.045.9464079
GCAGTGG271300.044.5414733
AGCAGTG286750.042.3708042
CAGTGGT286500.042.1947564
AGTGGTA304450.039.9074675
AGAGTAC212950.030.79880318-19
GCAGAGT222900.029.4025416-17
CAACGCA237300.028.05332612-13
TACATGG242850.026.9598222-23
CAGAGTA227100.026.30605518-19
AACGCAG230550.026.22759214-15
CATGGGG171750.025.46445324-25
TCAACGC272400.022.4479412-13
GAGTACA281900.021.5342720-21
CTTAGAG46150.020.46334686-87
ATCAACG334250.020.35882410-11
Produced by FastQC (version 0.11.2)