FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l01n01_sbc_index.35100000098f42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l01n01_sbc_index.35100000098f42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32891218
Sequences flagged as poor quality0
Sequence length101
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTT451950.1374074988648946No Hit
AGCAGCCGTCTAGCGCGCATTGACGTTTTTTTTTTTTTTTTTTTTTTTTT350980.1067093349963507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGCG63650.094.0586851
GATGCAG42200.093.872644
GCATGCT29450.093.384357
TGCTCGA36150.093.290095
CTGAGGA30550.093.287945
ATCCTCC986100.093.076294
CATCGTA23600.092.985554
CGCATGC14100.092.975996
AAGCTAG35450.092.85347
CATGCTA29850.092.769478
AGCTAGC693700.092.6907044
CTAGAGC11700.092.562364
GATATGC37050.092.561389
GTCGACT74000.092.429728
GTTCTCG69450.092.292511
CGTACTG15900.092.017572
TATCCTC1023900.091.9735643
CCACTAC635850.091.845811
CCGATAG17800.091.7960363
GATGCAC68000.091.505644
Produced by FastQC (version 0.11.2)