FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l01n01_perturseq_pilot_run_4.35100000098fd5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l01n01_perturseq_pilot_run_4.35100000098fd5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20285915
Sequences flagged as poor quality0
Sequence length101
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAG55250.076.686028
TGACTAG53900.075.372141
GACTAAC46100.074.6227341
CGTGAGC53400.073.853381
GTACTCC68050.073.385141
TGCTACC79750.073.2242051
GACCTGG62550.072.469381
GCGCGAT54900.072.1817251
CTGATCC63500.071.684511
CCTTACG53050.071.116211
CCGGTAG60300.070.3667761
GGCTCGA47700.070.0277941
CGCTATC43950.069.732351
TACGGGC61800.069.120161
CCTACCA50650.068.951131
GTAACTG61600.068.727491
CGTCTAC47250.068.582941
CTCTACG64250.068.555151
CTGCGGA66350.068.533761
CCGGGAT74900.068.005881
Produced by FastQC (version 0.11.2)