FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l01n01_perturseq_pilot_run_3.35100000098fbb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l01n01_perturseq_pilot_run_3.35100000098fbb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19856407
Sequences flagged as poor quality0
Sequence length101
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC45500.076.550551
TGACTAG55900.075.7393651
CGTGAGC48750.075.735961
AGACTAG54800.074.888298
CTGATCC60700.073.351111
CCTTACG49950.073.250571
TGCTACC82700.071.592631
CCGGTAG59950.070.6226961
GCGCGAT55950.070.491011
GACCTGG65100.070.364211
GTACTCC68350.070.077371
CGGACGT59200.069.189681
CGGCTAG70250.068.723161
GGCTCGA48500.068.5823061
CGCTATC42250.068.155621
GACGTGC59600.067.928041
GTAACTG58850.067.824811
GATCTAG49150.067.5786361
CCGGGAT75200.067.548491
GTTCTCG65550.067.416471
Produced by FastQC (version 0.11.2)