FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l01n01_perturseq_pilot_run_2.35100000098f92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l01n01_perturseq_pilot_run_2.35100000098f92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30433182
Sequences flagged as poor quality0
Sequence length101
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAG82250.076.921848
TGACTAG84200.076.8033451
CTGATCC95500.074.283191
GACTAAC70350.073.485041
CGTGAGC77850.073.363491
GACCTGG100150.073.2064061
GTACTCC102150.072.5173341
TGCTACC126350.071.038021
CCTTACG76950.070.763631
CCGGTAG91350.070.479831
GCGCGAT83450.070.376271
GACGTGC91950.068.986421
TACGGGC90400.068.4873051
GATCTAG75900.068.299431
CTAAGAC88000.068.195351
CTGCGGA93950.068.0236051
CGTCTAC71950.067.8224951
GACTGCG76650.067.25921
TCGGGAC107150.067.137911
GGCTCGA76200.067.032841
Produced by FastQC (version 0.11.2)