FastQCFastQC Report
Mon 14 Aug 2017
HTMNWBCXY_l01n01_perturseq_pilot_run_1.35100000098f78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTMNWBCXY_l01n01_perturseq_pilot_run_1.35100000098f78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17682127
Sequences flagged as poor quality0
Sequence length101
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAG49000.077.742958
TGACTAG48000.077.213951
CCGGTAG50500.073.203291
GTACTCC61000.072.5207441
CCTTACG45950.072.3860551
CTGATCC52050.071.9362641
CGTGAGC45400.071.797721
GACTAAC38900.071.579791
GACCTGG56450.071.295461
TGCTACC71900.070.712681
CGTCTAC42200.070.261031
GTAACTG52650.070.213991
GCGCGAT47650.069.404681
GACGTGC51100.068.903251
GATCTAG44900.068.575821
GTGACGC31600.068.241968
CCGGGAT63300.067.9340061
CCTACCA46250.067.704281
GATCGCG52100.066.9423141
ACGAGCC66700.066.893231
Produced by FastQC (version 0.11.2)