FastQCFastQC Report
Tue 29 Jun 2021
HTLWKAFX2_n01_ChIP-zmm16-GR.rep6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTLWKAFX2_n01_ChIP-zmm16-GR.rep6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12122366
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCGGGGGTTG937760.7735783592080951TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCGGGGGTGG835450.6891806434486469TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCGGGGTTTG281660.23234738169099992TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCGGGGGGGG272490.22478285179642327TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCGGGGTTGG178420.14718248896296318TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCGGGGGGTG127510.10518573684378116TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)