FastQCFastQC Report
Tue 29 Jun 2021
HTLWKAFX2_n01_ChIP-zmm16-GR.rep5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTLWKAFX2_n01_ChIP-zmm16-GR.rep5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9907020
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC5383985.434510074674321TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATTC136120.13739752216105347TruSeq Adapter, Index 23 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)