FastQCFastQC Report
Tue 29 Jun 2021
HTLWKAFX2_n01_ChIP-zmm16-GR.rep4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTLWKAFX2_n01_ChIP-zmm16-GR.rep4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9845004
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC188214719.11778806793781TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATTC280560.28497702997378166TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCGCGTATGC221210.22469264613808182TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGG168790.17144736558766255TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGGATGC117160.11900452249689285TruSeq Adapter, Index 7 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)