FastQCFastQC Report
Tue 29 Jun 2021
HTLWKAFX2_n01_ChIP-zmm16-GR.rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTLWKAFX2_n01_ChIP-zmm16-GR.rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21154378
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT293186013.859353368839301TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTTT826790.39083635548159346TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCGCGTAT815610.38555139744595657TruSeq Adapter, Index 27 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTGGTAT333570.15768367190942698TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCCTAT229550.10851181727016507TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCGCGTTT225330.10651695833363665TruSeq Adapter, Index 27 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)