FastQCFastQC Report
Tue 29 Jun 2021
HTLWKAFX2_n01_ChIP-zmm16-GR.rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTLWKAFX2_n01_ChIP-zmm16-GR.rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11824255
Sequences flagged as poor quality0
Sequence length151
%GC65

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGGGTTG166987414.122445769310623TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGGTTTG6805315.755381628694577TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGGGTGG3287012.779887612369659TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGGATTG2369472.0039063771882457TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGGTTGG1724021.4580368911191446TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGTTTTG1359001.149332452657694TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGGTTTG955760.8083046246888282TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGTTTTG814300.6886691804261663TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGGATGG789990.6681097456034228TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGGGTTG789580.6677630007133643TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGGATTG759170.6420446785019437TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGTTTGG735500.6220265039953892TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGTTTGG618220.522840551053745TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGTGTTG562360.4755986740813692TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGTATTG517870.43797262491378947TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC514100.4347842633637383TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGTATTG481940.4075859324752384TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGGTTGG446630.37772358596799543TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTGGGGTTG432610.365866602166479TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTGGGTTTG399820.33813546815423046TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGTATGG392570.3320040036349013TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATTG365140.3088059247707361TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGTATGC356560.30154965365682657TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGGATGG349560.2956296189485088TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTTTTG316630.26778008424209393TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGGGTTC283700.239930549535679TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGG278890.23586263997182064TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTGGTTTTG261440.22110483916322846TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGTATGG247270.2091209974751052TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTGGGATTG246900.20880808135480838TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGGTTTC231660.19591932007555654TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGGGTTT222610.18826556091694574TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGGGTGG220050.18610051965218952TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGGATTC203390.1720108370463932TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTTTGG200860.16987116735895835TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGGATTG195780.16557491359920773TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGGTTTG189240.16004390974315083TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATTC182650.15447061992489167TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGGGTTG172560.14593731275247362TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGTATTC169970.14374689991039605TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGGATTC167400.14157340145319938TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTGGTATTG158810.1343086731468494TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGGATGC144900.12254471846217796TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGGGTGTGG142520.1205319066613499TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGTTTGC124490.1052835886912114TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGGTTTC121070.10239122887657616TruSeq Adapter, Index 1 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)