FastQCFastQC Report
Wed 16 Jun 2021
HTLVYAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTLVYAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10767456
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTG682210.6335851291149924Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACG459350.4266095909748783No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTC358580.3330220248868442No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGG246070.22853123337583178No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTC194180.18033971998585366No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCT181940.1689721323216923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAA107850.10016293542318633Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)