FastQCFastQC Report
Wed 16 Jun 2021
HTLVYAFX2_n01_Ts_CA015840_837_sRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTLVYAFX2_n01_Ts_CA015840_837_sRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23451250
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT4066841.7341676882895367TruSeq Adapter, Index 6 (100% over 43bp)
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTG3763271.6047204306806673Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT2939561.2534768935557807TruSeq Adapter, Index 6 (100% over 43bp)
CTTCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC2774071.1829092265870689TruSeq Adapter, Index 6 (100% over 42bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTC1945180.8294568519801716No Hit
GACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC1903540.8117008688236236TruSeq Adapter, Index 6 (100% over 44bp)
ATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT1815620.7742103299397687TruSeq Adapter, Index 6 (100% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1710240.7292745589254304TruSeq Adapter, Index 6 (100% over 49bp)
AGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT1671970.7129555993816961TruSeq Adapter, Index 6 (100% over 43bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGG1597300.6811150791535633No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTC1535400.6547198976600394No Hit
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC1495420.6376717658973402TruSeq Adapter, Index 6 (100% over 44bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACG1361250.5804594637812484No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCT1129180.4815009860881616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAG1110940.47372314908586965Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA1104890.4711433292468419TruSeq Adapter, Index 6 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATT1070650.4565428282074516TruSeq Adapter, Index 6 (97% over 48bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT1064130.4537625926123341TruSeq Adapter, Index 6 (100% over 43bp)
CCGACCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT1017940.4340664143702361TruSeq Adapter, Index 6 (100% over 41bp)
CCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG1013150.4320238793241299TruSeq Adapter, Index 6 (100% over 45bp)
ACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG974920.41572197644048825TruSeq Adapter, Index 6 (100% over 45bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG969220.41329140237727197TruSeq Adapter, Index 6 (100% over 45bp)
CCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC967260.4124556260327275TruSeq Adapter, Index 6 (100% over 44bp)
CGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT953220.4064687383401737TruSeq Adapter, Index 6 (100% over 41bp)
TTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC931410.3971685944246042Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGGACGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA912220.3889856617451095TruSeq Adapter, Index 6 (100% over 38bp)
CCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG861780.36747721336815736TruSeq Adapter, Index 6 (100% over 45bp)
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT843700.35976760300623634TruSeq Adapter, Index 6 (100% over 43bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC798500.34049357710143385TruSeq Adapter, Index 6 (100% over 42bp)
CGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG761850.32486541229145566TruSeq Adapter, Index 6 (100% over 45bp)
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT723260.3084099994669794TruSeq Adapter, Index 6 (100% over 43bp)
TTGGCTGGGGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC721520.3076680347529449TruSeq Adapter, Index 5 (97% over 35bp)
TGGACGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT678700.2894088801236608TruSeq Adapter, Index 6 (100% over 39bp)
CGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC676000.28825755556740046TruSeq Adapter, Index 6 (100% over 44bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT652890.2784030701988167TruSeq Adapter, Index 6 (100% over 46bp)
CGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC646440.2756526837588615TruSeq Adapter, Index 6 (100% over 44bp)
GTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT581990.24817014018442513TruSeq Adapter, Index 6 (100% over 46bp)
AGGGTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT579870.24726613719950963TruSeq Adapter, Index 6 (100% over 39bp)
AAGTCTCTAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA574270.24487820478652522TruSeq Adapter, Index 6 (100% over 37bp)
GCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG571150.24354778529929108TruSeq Adapter, Index 6 (100% over 45bp)
AAGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC561160.23928788444112786TruSeq Adapter, Index 6 (100% over 42bp)
CTTCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC557210.23760353925696923TruSeq Adapter, Index 6 (100% over 42bp)
CTTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT549690.23439688715953308TruSeq Adapter, Index 6 (100% over 43bp)
CCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC546230.23292148606151056TruSeq Adapter, Index 6 (100% over 44bp)
CTTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC532440.22704120249453655TruSeq Adapter, Index 6 (100% over 42bp)
TCGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT519350.22145941047918555TruSeq Adapter, Index 6 (100% over 43bp)
CGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG510520.21769415276371196TruSeq Adapter, Index 6 (100% over 45bp)
TTGGACGTGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA509450.217237887106231TruSeq Adapter, Index 6 (100% over 37bp)
TTTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT503720.2147945205479452Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGACGTGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA498640.21262832471616652TruSeq Adapter, Index 6 (100% over 38bp)
TTGGACGTGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC482240.20563509407814082TruSeq Adapter, Index 6 (100% over 36bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTC472070.20129843824956026No Hit
CTTCGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT460500.19636479931773362TruSeq Adapter, Index 6 (100% over 41bp)
CGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG457820.19522200309151963TruSeq Adapter, Index 6 (100% over 45bp)
AACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC449100.1915036511913011TruSeq Adapter, Index 6 (100% over 44bp)
CGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG435450.18568306593465167TruSeq Adapter, Index 6 (100% over 45bp)
CCGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGA430570.18360215340333672Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG413350.17625926123340974TruSeq Adapter, Index 6 (100% over 45bp)
TCCGATATCGTTCAGCGGTTAGGATATAAGATCGGAAGAGCACACGTCTG409270.1745194819039497Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAA401610.1712531314961889Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC395880.1688097649379031TruSeq Adapter, Index 6 (100% over 44bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGT394840.1683662917754917No Hit
GCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC391210.16681839987207506TruSeq Adapter, Index 6 (100% over 44bp)
TTTCGATGAGGATTACCCCGACGGGCGGTCTGAAATATGACGATAGTCGG387800.1653643195991685No Hit
AGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC385210.16425990085816322TruSeq Adapter, Index 6 (100% over 44bp)
ACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC384420.16392303182133147TruSeq Adapter, Index 6 (100% over 44bp)
CGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC379670.16189755343531795TruSeq Adapter, Index 6 (100% over 44bp)
GGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT377620.16102339960556475TruSeq Adapter, Index 6 (100% over 46bp)
GACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC375170.15997867917488406TruSeq Adapter, Index 6 (100% over 44bp)
GCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT365630.15591066574276424TruSeq Adapter, Index 6 (100% over 46bp)
ATCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC365190.15572304248174404TruSeq Adapter, Index 6 (100% over 44bp)
AAGTCTCTAAGCAATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC361490.15414530142316507Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG356430.15198763392143275TruSeq Adapter, Index 6 (100% over 45bp)
GACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC355760.15170193486487926TruSeq Adapter, Index 6 (100% over 44bp)
GAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC354690.15124566920739832TruSeq Adapter, Index 6 (100% over 44bp)
TTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC354030.150964234315868TruSeq Adapter, Index 6 (100% over 44bp)
CGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT352190.15017962795160172TruSeq Adapter, Index 6 (100% over 46bp)
GAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG337730.1440136453280742TruSeq Adapter, Index 6 (100% over 45bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGATCGGAAGAGCACACGTCT335520.14307126485795Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGCTGGGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC326870.13938276211289377TruSeq Adapter, Index 6 (100% over 36bp)
CGGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC325780.13891796812536644TruSeq Adapter, Index 6 (100% over 42bp)
TGGACGTGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA320680.13674324396354137TruSeq Adapter, Index 6 (100% over 37bp)
CCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG318760.1359245242790896TruSeq Adapter, Index 6 (100% over 45bp)
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGC315620.13458557646180908No Hit
CGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG311620.1328799104525345TruSeq Adapter, Index 6 (100% over 45bp)
TTTTTATGACTGACAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC311600.13287138212248814Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT300670.1282106497521454TruSeq Adapter, Index 6 (100% over 43bp)
AGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT300380.128086988966473TruSeq Adapter, Index 6 (100% over 43bp)
CGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT300220.12801876232610201TruSeq Adapter, Index 6 (100% over 43bp)
GATCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT293180.12501679014977882TruSeq Adapter, Index 6 (100% over 43bp)
CGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT291420.12426629710569799TruSeq Adapter, Index 6 (100% over 43bp)
ATTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT290830.12401471136933001TruSeq Adapter, Index 6 (100% over 43bp)
CGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC284170.12117477746388784TruSeq Adapter, Index 6 (100% over 44bp)
GACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG278920.11893609082671498TruSeq Adapter, Index 6 (100% over 45bp)
CCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC277630.11838601353872395TruSeq Adapter, Index 6 (100% over 44bp)
CGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC273730.11672298917968126TruSeq Adapter, Index 6 (100% over 44bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAAGATCGGAAGAGCACACGTC272070.1160151377858323No Hit
GACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT271760.11588294867011352TruSeq Adapter, Index 6 (100% over 43bp)
TTCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT255390.1089025105271574TruSeq Adapter, Index 6 (100% over 43bp)
CGGAGAGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT252190.10753797771973776TruSeq Adapter, Index 6 (100% over 39bp)
TCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC250320.10674057886040189TruSeq Adapter, Index 6 (100% over 44bp)
GGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG248360.10590480251585738TruSeq Adapter, Index 6 (100% over 45bp)
GGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG246570.105141516976707TruSeq Adapter, Index 6 (100% over 45bp)
CCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGT244610.10430574063216246TruSeq Adapter, Index 6 (100% over 46bp)
TTCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC243690.10391343745002932TruSeq Adapter, Index 6 (100% over 42bp)
AGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA238380.10164916582271732TruSeq Adapter, Index 6 (100% over 40bp)
CCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC235600.10046372794627152TruSeq Adapter, Index 6 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)