FastQCFastQC Report
Wed 16 Jun 2021
HTLVYAFX2_n01_Ts_CA015840_836_sRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTLVYAFX2_n01_Ts_CA015840_836_sRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19468131
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACG3729221.915551112739071No Hit
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTG2688071.3807540128017426Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATT2380711.222875477877152TruSeq Adapter, Index 5 (97% over 48bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTC1979151.016610171772524No Hit
CCGACCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT1975531.0147507226040342TruSeq Adapter, Index 5 (100% over 41bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGG1676390.8610944728078931No Hit
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT1513400.7773730308266366TruSeq Adapter, Index 5 (100% over 43bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTC1198790.6157704609651538No Hit
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT1011160.5193924367983758TruSeq Adapter, Index 5 (100% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATT974570.5005976177168728TruSeq Adapter, Index 5 (97% over 48bp)
GACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC889520.45691083545718897TruSeq Adapter, Index 5 (100% over 44bp)
TTGGACGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT886650.4554366312821708TruSeq Adapter, Index 5 (100% over 38bp)
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAG833830.4283051105419416Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC826200.4243858848083568Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGACGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG775090.3981327226532429TruSeq Adapter, Index 5 (100% over 39bp)
ATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT728900.3744067676553029TruSeq Adapter, Index 5 (100% over 43bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCT720190.36993278913111893Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATT717260.3684277653566231TruSeq Adapter, Index 5 (97% over 41bp)
AGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT691020.3549493271850287TruSeq Adapter, Index 5 (100% over 43bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC690650.35475927298824933TruSeq Adapter, Index 5 (100% over 44bp)
AGGGTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG612640.31468865706728605TruSeq Adapter, Index 5 (100% over 39bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTC612410.31457051526928803No Hit
TGGACGTGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT589970.3030439850646166TruSeq Adapter, Index 5 (100% over 38bp)
TTGGCTGGGGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC567210.29135308366273066TruSeq Adapter, Index 5 (100% over 35bp)
CGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT565300.2903719930793562TruSeq Adapter, Index 5 (100% over 41bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGT547930.28144971903055305TruSeq Adapter, Index 5 (97% over 43bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTCAGATCGGAAGAGCACACG540270.2775150834972294No Hit
CTTCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATG525140.2697434078289282TruSeq Adapter, Index 5 (97% over 41bp)
TTGGACGTGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG491750.25259230071957084TruSeq Adapter, Index 5 (100% over 37bp)
TTGGACGTGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA478830.2459558136320328TruSeq Adapter, Index 5 (100% over 36bp)
TTTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT430920.2213463634490645Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCG427630.21965642207770228TruSeq Adapter, Index 5 (100% over 45bp)
CTTCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC415450.21340004338372287TruSeq Adapter, Index 5 (100% over 42bp)
CCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC402990.2069998398921807TruSeq Adapter, Index 5 (100% over 44bp)
GACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTC382930.19669582046679263TruSeq Adapter, Index 5 (97% over 44bp)
GTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT382390.19641844407149306TruSeq Adapter, Index 5 (97% over 46bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT376550.1934186697223272TruSeq Adapter, Index 5 (100% over 43bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGT375450.19285364373190214TruSeq Adapter, Index 5 (97% over 43bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT371430.1907887305668942TruSeq Adapter, Index 5 (97% over 46bp)
CCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCG366350.18817933781111293TruSeq Adapter, Index 5 (100% over 45bp)
CTTCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA364640.18730097922599762TruSeq Adapter, Index 5 (100% over 40bp)
CCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCG363160.18654076243888024TruSeq Adapter, Index 5 (100% over 45bp)
TGGACGTGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG358830.18431661467657065TruSeq Adapter, Index 5 (100% over 37bp)
CCGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGA356430.18308383069746142Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG342080.17571280982237072TruSeq Adapter, Index 5 (97% over 49bp)
TTTCGATGAGGATTACCCCGACGGGCGGTCTGAAATATGACGATAGTCGG338930.17409478084978985No Hit
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT324580.16672375997469915TruSeq Adapter, Index 5 (100% over 43bp)
AAGTCTCTAAGCAATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC324360.16661075477661413Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG323640.16624091958288137TruSeq Adapter, Index 5 (100% over 49bp)
CGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAA316560.1626042068445091Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGTATT314290.16143819866426828TruSeq Adapter, Index 5 (97% over 41bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCG309630.15904454310483115TruSeq Adapter, Index 5 (100% over 45bp)
ACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCG306620.15749842653103166TruSeq Adapter, Index 5 (97% over 45bp)
CCGACCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT301810.1550277219729002TruSeq Adapter, Index 5 (100% over 41bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTC297830.15298335520754408TruSeq Adapter, Index 5 (97% over 44bp)
TCCGATATCGTTCAGCGGTTAGGATATAAGATCGGAAGAGCACACGTCTG290290.14911035887317586Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGT279280.1434549623690122TruSeq Adapter, Index 5 (97% over 43bp)
CGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCG274790.14114862900809533TruSeq Adapter, Index 5 (100% over 45bp)
CCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCG274430.14096371141122896TruSeq Adapter, Index 5 (97% over 45bp)
TTTTTATGACTGACAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC274170.14083015981349212Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGT268740.13804098606075746No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGATCGGAAGAGCACACGTCT265530.13639213748869883Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCG263360.13527749530758756TruSeq Adapter, Index 5 (97% over 45bp)
CGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC263250.13522099270854507TruSeq Adapter, Index 5 (100% over 44bp)
CGGAGAGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG262740.13495902611298433TruSeq Adapter, Index 5 (100% over 39bp)
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGC251550.12921117081038752No Hit
TTGGCTGGGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA251430.12914953161143203TruSeq Adapter, Index 5 (100% over 36bp)
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT251320.12909302901238953TruSeq Adapter, Index 5 (100% over 43bp)
CGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC250890.12887215521613246TruSeq Adapter, Index 5 (100% over 44bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATG245120.12590833706635732TruSeq Adapter, Index 5 (97% over 41bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCG242270.12444440609116508TruSeq Adapter, Index 5 (97% over 45bp)
AGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGT235590.12101315734931103TruSeq Adapter, Index 5 (97% over 43bp)
GCACCGCTGGTCTAATGGTAGAATTTCACAAGATCGGAAGAGCACACGTC225700.11593306003539836No Hit
CGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA225220.11568650323957651TruSeq Adapter, Index 5 (100% over 40bp)
GCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCG219650.11282541708806047TruSeq Adapter, Index 5 (100% over 45bp)
CGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT217170.1115515403096476TruSeq Adapter, Index 5 (97% over 46bp)
GGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT210950.10835657516378948TruSeq Adapter, Index 5 (97% over 46bp)
TGGCTGGGGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA210870.10831548236448582TruSeq Adapter, Index 5 (100% over 36bp)
CGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCG208670.1071854303836357TruSeq Adapter, Index 5 (97% over 45bp)
CCGACCATAGCTCAGTTGGAAGAGCATGAGATCGGAAGAGCACACGTCTG207900.10678991219033815Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTGGTATT205830.10572663600835641TruSeq Adapter, Index 5 (97% over 43bp)
CCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC205650.10563417720992324TruSeq Adapter, Index 5 (100% over 44bp)
GCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGT203670.1046171304271581TruSeq Adapter, Index 5 (97% over 46bp)
AACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC203310.10443221283029172TruSeq Adapter, Index 5 (100% over 44bp)
AGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA202230.10387746003969256TruSeq Adapter, Index 5 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)