FastQCFastQC Report
Wed 16 Jun 2021
HTLVYAFX2_n01_Ts_CA015840_835_sRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTLVYAFX2_n01_Ts_CA015840_835_sRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9318162
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1624061.7428973653817137TruSeq Adapter, Index 4 (100% over 49bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT1195231.2826885817181544TruSeq Adapter, Index 4 (100% over 43bp)
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTG1089271.1689751691374328Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTC923180.9907318632150848No Hit
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACG856600.919280003932106No Hit
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT852630.9150195070658784TruSeq Adapter, Index 4 (100% over 43bp)
GACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC728330.781624101405406TruSeq Adapter, Index 4 (100% over 44bp)
CTTCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC685560.7357244915896505TruSeq Adapter, Index 4 (100% over 42bp)
CCGACCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT660820.7091741912192554TruSeq Adapter, Index 4 (100% over 41bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTC655740.7037224723073069No Hit
ATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT587330.6303067064084097TruSeq Adapter, Index 4 (100% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATT540600.5801573314565683TruSeq Adapter, Index 4 (97% over 48bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC463900.4978449612702591TruSeq Adapter, Index 4 (100% over 44bp)
AGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT462210.4960312988763235TruSeq Adapter, Index 4 (100% over 43bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCT446990.47969760560076113Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGACGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC436260.4681824591587912TruSeq Adapter, Index 4 (100% over 38bp)
TTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC402540.43199506512121166Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG399790.4290438393322632TruSeq Adapter, Index 4 (100% over 45bp)
CCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG371950.3991667026179626TruSeq Adapter, Index 4 (100% over 45bp)
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAG371110.3982652372860656Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG364310.39096766078975664TruSeq Adapter, Index 4 (100% over 45bp)
TGGACGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA354500.3804398335208166TruSeq Adapter, Index 4 (100% over 39bp)
CCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC351320.377027143335778TruSeq Adapter, Index 4 (100% over 44bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGG310290.3329948545646663No Hit
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG307010.32947484707821134TruSeq Adapter, Index 4 (100% over 45bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT304800.32710313471691094TruSeq Adapter, Index 4 (100% over 43bp)
CGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG302300.3244202021815032TruSeq Adapter, Index 4 (100% over 45bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTC286810.3077967521921169No Hit
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGT285620.3065196763052628TruSeq Adapter, Index 4 (100% over 46bp)
TGGACGTGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC274870.29498306640300953TruSeq Adapter, Index 4 (100% over 38bp)
AGGGTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA270110.28987476285559316TruSeq Adapter, Index 4 (100% over 39bp)
GTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGT269080.2887693946510052TruSeq Adapter, Index 4 (100% over 46bp)
TTGGCTGGGGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG268580.28823280814392366TruSeq Adapter, Index 2 (97% over 35bp)
CGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT268010.2876210995258507TruSeq Adapter, Index 4 (100% over 41bp)
CTTCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA265920.2853781679262498TruSeq Adapter, Index 4 (100% over 40bp)
TTGGACGTGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC263990.28330694400891504TruSeq Adapter, Index 4 (100% over 37bp)
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT248170.2663293469248549TruSeq Adapter, Index 4 (100% over 43bp)
TTGGACGTGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA230180.24702296440006088TruSeq Adapter, Index 2 (97% over 36bp)
GCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG229580.246379060591563TruSeq Adapter, Index 4 (100% over 45bp)
CGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC222990.23930685042822822TruSeq Adapter, Index 4 (100% over 44bp)
TTTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT216780.23264244601027542Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC212780.22834975395362306TruSeq Adapter, Index 4 (100% over 42bp)
CGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC209380.22470096570546852TruSeq Adapter, Index 4 (100% over 44bp)
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT201590.21634094792513803TruSeq Adapter, Index 4 (100% over 43bp)
CGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG188660.20246482085200923TruSeq Adapter, Index 4 (100% over 45bp)
CGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG185820.19941700949178603TruSeq Adapter, Index 4 (100% over 45bp)
CGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAA184130.19760334709785043Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT183460.19688432117836116TruSeq Adapter, Index 4 (100% over 43bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG181680.19497407321315083TruSeq Adapter, Index 4 (100% over 45bp)
GGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGT178020.19104625998131392TruSeq Adapter, Index 4 (100% over 46bp)
CCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC172210.18481112476902634TruSeq Adapter, Index 4 (100% over 44bp)
GCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGT171900.18447844113463577TruSeq Adapter, Index 4 (100% over 46bp)
CGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGT166370.17854379436631387TruSeq Adapter, Index 4 (100% over 46bp)
CGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG164690.17674086370251987TruSeq Adapter, Index 4 (100% over 45bp)
AACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC163560.1755281781965156TruSeq Adapter, Index 4 (100% over 44bp)
AAGTCTCTAAGCAATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC163420.17537793397453275Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGACGTGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC160730.17249109856643402TruSeq Adapter, Index 4 (100% over 37bp)
CGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC157380.16889596896898768TruSeq Adapter, Index 4 (100% over 44bp)
CCGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGA153730.16497888746729236Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG153630.16487157016587606TruSeq Adapter, Index 4 (100% over 45bp)
CTTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC152670.16384132407227947TruSeq Adapter, Index 4 (100% over 42bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGT151400.16247839434429237No Hit
CTTCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC144860.15545984283166572TruSeq Adapter, Index 4 (100% over 42bp)
TTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC143740.15425788905580307TruSeq Adapter, Index 4 (100% over 44bp)
AAGTCTCTAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC140830.15113495558458848TruSeq Adapter, Index 4 (100% over 37bp)
AGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC139620.14983641623745111TruSeq Adapter, Index 4 (100% over 44bp)
TTTTTATGACTGACAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC139350.14954665952362708Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGATCGGAAGAGCACACGTCT137970.14806568076408202Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGCTGGGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA137500.14756128944742536TruSeq Adapter, Index 2 (97% over 36bp)
TCGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT136540.1465310433538288TruSeq Adapter, Index 4 (100% over 43bp)
CGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG136410.14639153086198758TruSeq Adapter, Index 4 (100% over 45bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTCAGATCGGAAGAGCACACG129690.1391798082068116No Hit
ACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC129380.13884712457242104TruSeq Adapter, Index 4 (100% over 44bp)
GACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC128400.1377954150185412TruSeq Adapter, Index 4 (100% over 44bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG127480.13680809584551115TruSeq Adapter, Index 4 (100% over 45bp)
ATCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC126920.13620711895757984TruSeq Adapter, Index 4 (100% over 44bp)
CTTCGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT125640.13483345749945105TruSeq Adapter, Index 4 (100% over 41bp)
AAGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC121580.13047637506194892TruSeq Adapter, Index 4 (100% over 42bp)
GACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC120230.12902759149282875TruSeq Adapter, Index 4 (100% over 44bp)
TTTCGATGAGGATTACCCCGACGGGCGGTCTGAAATATGACGATAGTCGG118980.12768612522512487No Hit
TCCGATATCGTTCAGCGGTTAGGATATAAGATCGGAAGAGCACACGTCTG118720.12740710024144247Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC117910.12653783009997036TruSeq Adapter, Index 4 (100% over 44bp)
CCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG116060.12455246002376864TruSeq Adapter, Index 4 (100% over 45bp)
GACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG113990.12233099188445104TruSeq Adapter, Index 4 (100% over 45bp)
AGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC109220.11721195660689308TruSeq Adapter, Index 4 (100% over 44bp)
CCGACCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT109190.11717976141646819TruSeq Adapter, Index 4 (100% over 41bp)
CGGAGAGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA108020.11592414898989736TruSeq Adapter, Index 4 (100% over 39bp)
GCACCGCTGGTCTAATGGTAGAATTTCACAAGATCGGAAGAGCACACGTC106740.11455048753176861No Hit
CCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGT106540.11433585292893599TruSeq Adapter, Index 4 (100% over 46bp)
AGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA103980.11158853001267846TruSeq Adapter, Index 4 (100% over 40bp)
CGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA102470.1099680387612922TruSeq Adapter, Index 4 (100% over 40bp)
TCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG99360.10663047068724497TruSeq Adapter, Index 4 (100% over 45bp)
TTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA97050.10415144102452822Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT96690.10376509873942952TruSeq Adapter, Index 4 (100% over 43bp)
GCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC95630.10262753534441664TruSeq Adapter, Index 4 (100% over 44bp)
GGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG95590.10258460842385012TruSeq Adapter, Index 4 (100% over 45bp)
CGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC94620.10154363060011191TruSeq Adapter, Index 4 (100% over 44bp)
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGC94370.10127533734657114No Hit
CGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC93560.10040606720509904TruSeq Adapter, Index 4 (100% over 44bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAAGATCGGAAGAGCACACGTC93330.10015923741184152No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)