FastQCFastQC Report
Wed 16 Jun 2021
HTLVYAFX2_n01_Ts_BTPI3_885_sRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTLVYAFX2_n01_Ts_BTPI3_885_sRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23641669
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC4329151.831152445286329TruSeq Adapter, Index 3 (100% over 42bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT3607041.5257129266127532TruSeq Adapter, Index 3 (100% over 43bp)
CTTCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA3605091.524888111748794TruSeq Adapter, Index 3 (100% over 40bp)
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTG3330671.4088133963807714Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT3241661.371163770205902TruSeq Adapter, Index 3 (100% over 43bp)
CGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT2564121.0845765584485596TruSeq Adapter, Index 3 (100% over 41bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTC2534491.0720436023361972No Hit
ATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT2226850.9419174255421646TruSeq Adapter, Index 3 (100% over 43bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGG1645160.6958730367132709No Hit
GGAAACTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA1484970.6281155530939884TruSeq Adapter, Index 3 (100% over 40bp)
TCGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT1467210.6206033931022382TruSeq Adapter, Index 3 (100% over 43bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC1093090.46235737417692463TruSeq Adapter, Index 3 (100% over 42bp)
CTTCGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA1033440.43712649897940786TruSeq Adapter, Index 3 (100% over 40bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCT1017230.43026996105900983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT1011430.4278166655662085TruSeq Adapter, Index 3 (100% over 43bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT985460.4168318235062TruSeq Adapter, Index 3 (100% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATT907710.3839449744432172TruSeq Adapter, Index 3 (97% over 48bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCG884140.3739752891388506TruSeq Adapter, Index 3 (100% over 45bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGT755020.3193598556853156No Hit
CTTCGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT738220.31225375839582226TruSeq Adapter, Index 3 (100% over 41bp)
CTTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT714510.30222485561404316TruSeq Adapter, Index 3 (100% over 43bp)
CGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT708880.29984346705809983TruSeq Adapter, Index 3 (100% over 41bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC693980.29354103553348965TruSeq Adapter, Index 3 (100% over 44bp)
CTTCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA670910.28378284121988173TruSeq Adapter, Index 3 (100% over 40bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACG653720.27651178095759654No Hit
CCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCG633000.26774759430055467TruSeq Adapter, Index 3 (100% over 45bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTC614980.26012545899361No Hit
CGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC583490.24680575639562502TruSeq Adapter, Index 3 (100% over 44bp)
TTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC552700.2337821411847023Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAA547440.23155725596192045Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTCGGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA540770.22873596614519898TruSeq Adapter, Index 3 (100% over 40bp)
CTTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC537310.22727244848914854TruSeq Adapter, Index 3 (100% over 42bp)
CTTCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATG527210.22300033047582216TruSeq Adapter, Index 3 (97% over 41bp)
GCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC511300.21627068714987932TruSeq Adapter, Index 3 (100% over 44bp)
CGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCG469360.19853082284503687TruSeq Adapter, Index 3 (100% over 45bp)
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAG469340.19852236320540653Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCG463720.19614520446927836TruSeq Adapter, Index 3 (100% over 45bp)
CGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCG456590.19312934294105885TruSeq Adapter, Index 3 (100% over 45bp)
CGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCG436440.18460625601348196TruSeq Adapter, Index 3 (100% over 45bp)
CGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT433770.18347689412283033TruSeq Adapter, Index 3 (100% over 43bp)
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT421140.17813463169626476TruSeq Adapter, Index 3 (100% over 43bp)
GACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC414660.17539370845603158TruSeq Adapter, Index 3 (100% over 44bp)
GGAAACTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA412960.17467463908745193TruSeq Adapter, Index 3 (100% over 40bp)
CTTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC408940.1729742515217517TruSeq Adapter, Index 3 (100% over 42bp)
TCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT407830.17250474152226733TruSeq Adapter, Index 3 (100% over 43bp)
AAGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC405060.17133308143346393TruSeq Adapter, Index 3 (100% over 42bp)
CGGACGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT401170.169687681525361TruSeq Adapter, Index 3 (100% over 41bp)
CTTCGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA398400.16851602143655764TruSeq Adapter, Index 3 (100% over 40bp)
AGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT385430.1630299451362761TruSeq Adapter, Index 3 (100% over 43bp)
TTCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT376400.1592104178431734TruSeq Adapter, Index 3 (100% over 43bp)
CCGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGA357780.15133449334731824Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC357610.15126258641046028TruSeq Adapter, Index 3 (100% over 44bp)
CCGACCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT349890.14799716551314546TruSeq Adapter, Index 3 (100% over 41bp)
CGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC348950.14759956245051903TruSeq Adapter, Index 3 (100% over 44bp)
CGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT346570.14659286533450747TruSeq Adapter, Index 3 (100% over 43bp)
CTTCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC332480.14063304921492642TruSeq Adapter, Index 3 (100% over 42bp)
CCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCG321380.13593794922008257TruSeq Adapter, Index 3 (100% over 45bp)
AGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC320350.13550227777911958TruSeq Adapter, Index 3 (100% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG316790.1339964619249174TruSeq Adapter, Index 3 (100% over 49bp)
ATTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT315290.1333619889526412TruSeq Adapter, Index 3 (100% over 43bp)
GCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCG302750.12805779490441221TruSeq Adapter, Index 3 (100% over 45bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCG299080.12650545103224312TruSeq Adapter, Index 3 (100% over 45bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGT297190.1257060150871751TruSeq Adapter, Index 3 (100% over 46bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAAGATCGGAAGAGCACACGTC292840.12386604346757413No Hit
AACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC288030.12183150013647515TruSeq Adapter, Index 3 (100% over 44bp)
TCCGATATCGTTCAGCGGTTAGGATATGCAGATCGGAAGAGCACACGTCT286730.12128162356050243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATAAGATCGGAAGAGCACACGTCTG285380.12071059788545387Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT284260.1202368580661543TruSeq Adapter, Index 3 (100% over 43bp)
CCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC282800.1196193043731388TruSeq Adapter, Index 3 (100% over 44bp)
CGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT280860.11879871932899491TruSeq Adapter, Index 3 (100% over 43bp)
AGGAAACTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC280850.11879448950917973TruSeq Adapter, Index 3 (100% over 39bp)
CGGAGAGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC276440.11692913897068773TruSeq Adapter, Index 3 (100% over 39bp)
CGAACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC264430.11184912537266299TruSeq Adapter, Index 3 (100% over 42bp)
TCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC261610.11065631618478373TruSeq Adapter, Index 3 (100% over 44bp)
TCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC260690.11026717276178767TruSeq Adapter, Index 3 (100% over 42bp)
ACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC253600.10726823051282884TruSeq Adapter, Index 3 (100% over 44bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCG246730.10436234429980387TruSeq Adapter, Index 3 (100% over 45bp)
CCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCG245710.10393090267865605TruSeq Adapter, Index 3 (100% over 45bp)
CCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC245440.10381669754364635TruSeq Adapter, Index 3 (100% over 42bp)
TTTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT239540.10132110385269331Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC236890.10020020160167203TruSeq Adapter, Index 3 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)