FastQCFastQC Report
Wed 16 Jun 2021
HTLVYAFX2_n01_Ts_BTPI3_884_sRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTLVYAFX2_n01_Ts_BTPI3_884_sRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32663133
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT9963753.0504575295946044TruSeq Adapter, Index 2 (100% over 41bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACG7238852.216214225377584No Hit
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTG6812242.0856051989868822Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT5049281.5458651807834847TruSeq Adapter, Index 2 (100% over 43bp)
CTTCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA4928741.5089611887506322TruSeq Adapter, Index 2 (100% over 40bp)
CTTCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC4357191.3339779744949758TruSeq Adapter, Index 2 (100% over 42bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT3882231.1885663264451698TruSeq Adapter, Index 2 (100% over 43bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTC2751290.8423227496272327No Hit
CCGACCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT2705410.8282763322183454TruSeq Adapter, Index 2 (100% over 41bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC2619620.8020112461349007TruSeq Adapter, Index 2 (100% over 42bp)
ATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT2355850.7212565922564745TruSeq Adapter, Index 2 (100% over 43bp)
GGAAACTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA2122690.649873360280534TruSeq Adapter, Index 2 (100% over 40bp)
CGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT1781120.5452998032981099TruSeq Adapter, Index 2 (100% over 41bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGG1762790.5396879717570264No Hit
TCGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT1678760.5139617194713073TruSeq Adapter, Index 2 (100% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATT1495080.457727064945056TruSeq Adapter, Index 2 (97% over 48bp)
GACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC1327400.40639089948903556TruSeq Adapter, Index 2 (100% over 44bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT1237190.37877260579993965TruSeq Adapter, Index 2 (100% over 43bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG1218470.37304137358776945TruSeq Adapter, Index 2 (100% over 45bp)
CGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAA1183640.3623779751930104Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTCAGATCGGAAGAGCACACG1181110.36160340160878013No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCT1142070.34965108827741664Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC1103500.33784266806249114TruSeq Adapter, Index 2 (100% over 44bp)
ACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG963090.2948553649155456TruSeq Adapter, Index 2 (100% over 45bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTC941510.2882485277820716No Hit
CTTCGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA928010.2841154276290643TruSeq Adapter, Index 2 (100% over 40bp)
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT911960.2792016307804888TruSeq Adapter, Index 2 (100% over 43bp)
CCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG886800.27149875671755064TruSeq Adapter, Index 2 (100% over 45bp)
AAGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC770770.23597552629136953TruSeq Adapter, Index 2 (100% over 42bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGT741050.22687658284341553TruSeq Adapter, Index 2 (100% over 46bp)
CTTCGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT688580.21081260024872692TruSeq Adapter, Index 2 (100% over 41bp)
AGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT687320.21042684423444621TruSeq Adapter, Index 2 (100% over 43bp)
TCCGATATCGTTCAGCGGTTAGGATATAAGATCGGAAGAGCACACGTCTG677060.20728568811816062Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG672880.2060059578485628TruSeq Adapter, Index 2 (100% over 45bp)
CCGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA661910.20264743127978568TruSeq Adapter, Index 2 (100% over 40bp)
CGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC660520.20222187504180936TruSeq Adapter, Index 2 (100% over 44bp)
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAG659850.20201675081199344Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG657560.20131565456381664TruSeq Adapter, Index 2 (100% over 45bp)
GCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC654910.20050434231155967TruSeq Adapter, Index 2 (100% over 44bp)
CGGACGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT644410.1972897088592206TruSeq Adapter, Index 2 (100% over 41bp)
TCCGATACCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTG633360.19390668984509232Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGA619670.18971542013437598Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC617430.18902963166454362Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGT589320.18042359867928162TruSeq Adapter, Index 2 (100% over 46bp)
CGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT586720.17962759420536908TruSeq Adapter, Index 2 (100% over 43bp)
AGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC583190.17854686505424938TruSeq Adapter, Index 2 (100% over 42bp)
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT582500.1783356177130957TruSeq Adapter, Index 2 (100% over 43bp)
TTGGACGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG575060.1760578202954383TruSeq Adapter, Index 2 (100% over 38bp)
CTTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT556430.17035414208428812TruSeq Adapter, Index 2 (100% over 43bp)
CTTCGGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA543980.16654250527651465TruSeq Adapter, Index 2 (100% over 40bp)
GGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGT534960.16378098206317196TruSeq Adapter, Index 2 (100% over 46bp)
GCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG501480.1535308936837137TruSeq Adapter, Index 2 (100% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTATT497820.15241036430889834TruSeq Adapter, Index 2 (97% over 42bp)
TCCGATACCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTC484510.14833543371359997No Hit
CCGACCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT481710.14747819812630955TruSeq Adapter, Index 2 (100% over 41bp)
CGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG477990.14633929941748086TruSeq Adapter, Index 2 (100% over 45bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGT474550.1452861242673812No Hit
CGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG469080.143611453316496TruSeq Adapter, Index 2 (100% over 45bp)
GACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT450950.13806085288879055TruSeq Adapter, Index 2 (100% over 43bp)
CCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC438870.1343624936407662TruSeq Adapter, Index 2 (100% over 42bp)
CTTCGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA438160.13414512318827468TruSeq Adapter, Index 2 (100% over 40bp)
TTGGACGTGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT432590.13243983668070053TruSeq Adapter, Index 2 (100% over 37bp)
CGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGT427150.130774350396822TruSeq Adapter, Index 2 (100% over 46bp)
CGGACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC415670.1272596844889313TruSeq Adapter, Index 2 (100% over 42bp)
TCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC415170.12710660670548657TruSeq Adapter, Index 2 (100% over 42bp)
CTTCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA413610.12662900402113905TruSeq Adapter, Index 2 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATT409190.12527579641548775TruSeq Adapter, Index 2 (97% over 48bp)
CGGACGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT406980.1245991926126621TruSeq Adapter, Index 2 (100% over 41bp)
AGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC403170.12343273990281335TruSeq Adapter, Index 2 (100% over 44bp)
GGAAACTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA403160.12342967834714448TruSeq Adapter, Index 2 (100% over 40bp)
GCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGT393990.12062223179876835TruSeq Adapter, Index 2 (100% over 46bp)
AGGAAACTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT388300.11888020662316748TruSeq Adapter, Index 2 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATT386770.11841178860582664TruSeq Adapter, Index 2 (97% over 48bp)
CCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC377870.11568700406051066TruSeq Adapter, Index 2 (100% over 44bp)
AACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC374510.11465832135576218TruSeq Adapter, Index 2 (100% over 44bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAAGATCGGAAGAGCACACGTC357960.10959144672374203No Hit
GAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG356630.10918425981977908TruSeq Adapter, Index 2 (100% over 45bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG355730.1089087198095786TruSeq Adapter, Index 2 (100% over 45bp)
ATTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT353100.10810353066865938TruSeq Adapter, Index 2 (100% over 43bp)
CGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC340100.10412350829909671TruSeq Adapter, Index 2 (100% over 44bp)
CCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG338770.10371632139513377TruSeq Adapter, Index 2 (100% over 45bp)
TTCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT337840.1034315967179266TruSeq Adapter, Index 2 (100% over 41bp)
TTGGCTGGGGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG331830.1015916017609211TruSeq Adapter, Index 2 (100% over 35bp)
AGATTGATTTAGTACGAGAGGAACTAGATCGGAAGAGCACACGTCTGAAC327690.10032411771399884Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)