FastQCFastQC Report
Wed 16 Jun 2021
HTLVYAFX2_n01_Ts_BTPI3_883_sRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTLVYAFX2_n01_Ts_BTPI3_883_sRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34416842
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC5063771.4713058217253054TruSeq Adapter, Index 1 (100% over 42bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT4995631.4515073753716277TruSeq Adapter, Index 1 (100% over 43bp)
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTG4798541.3942418075429466Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT4657861.353366470985339TruSeq Adapter, Index 1 (100% over 43bp)
CTTCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA3781851.0988370170627508TruSeq Adapter, Index 1 (100% over 40bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTC2816730.8184161696183514No Hit
ATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT2811400.8168675092270231TruSeq Adapter, Index 1 (100% over 43bp)
CGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT2061850.5990816937823639TruSeq Adapter, Index 1 (100% over 41bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCT1862690.5412146762332233Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAACTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA1681060.4884410952056554TruSeq Adapter, Index 1 (100% over 40bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT1443070.41929181067803956TruSeq Adapter, Index 1 (100% over 43bp)
TCGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT1432540.4162322621000497TruSeq Adapter, Index 1 (100% over 43bp)
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT1428200.41497125157502823TruSeq Adapter, Index 1 (100% over 43bp)
GCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCG1365330.3967040322874481TruSeq Adapter, Index 1 (100% over 45bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC1204450.3499594762355012TruSeq Adapter, Index 1 (100% over 42bp)
CTTCGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA1196450.3476350328714064TruSeq Adapter, Index 1 (100% over 40bp)
CCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCG1110760.3227373388877457TruSeq Adapter, Index 1 (100% over 45bp)
CTTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT1104870.32102596746093087TruSeq Adapter, Index 1 (100% over 43bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACG1078920.31348605429864834No Hit
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC1020230.29643335666880766TruSeq Adapter, Index 1 (100% over 44bp)
CTTCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA1013850.29457961308594205TruSeq Adapter, Index 1 (100% over 40bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGG1007860.29283918611707604No Hit
ACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCG961280.279305114629634TruSeq Adapter, Index 1 (100% over 45bp)
CGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC923300.26826981975859376TruSeq Adapter, Index 1 (100% over 44bp)
CGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAA921030.26761025895403184Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTCGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT898590.2610901953177459TruSeq Adapter, Index 1 (100% over 41bp)
CTTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC869390.2526059770387998TruSeq Adapter, Index 1 (100% over 42bp)
CGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCG723440.21019941341509485TruSeq Adapter, Index 1 (100% over 45bp)
AAGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC720350.2093015971657132TruSeq Adapter, Index 1 (100% over 42bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTC714120.2074914368959244No Hit
TTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC713130.20720378702961764Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGT695520.20208710607440392TruSeq Adapter, Index 1 (100% over 46bp)
AGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT665070.193239693519818TruSeq Adapter, Index 1 (100% over 43bp)
CGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCG662550.19250749386012814TruSeq Adapter, Index 1 (100% over 45bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGT658250.19125810555192718No Hit
CGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT649580.1887389900560894TruSeq Adapter, Index 1 (100% over 41bp)
GCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC646990.1879864515169637TruSeq Adapter, Index 1 (100% over 44bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGATCGGAAGAGCACACGTCT617220.17933661664832584Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGA616100.17901119457735257Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATCGTTCAGCGGTTAGGATATGCAGATCGGAAGAGCACACGTCT599320.17413567462116367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT597490.17360395820162697TruSeq Adapter, Index 1 (100% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG578850.16818800516328605TruSeq Adapter, Index 1 (100% over 49bp)
CGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCG566100.1644834235517599TruSeq Adapter, Index 1 (100% over 45bp)
CCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCG552620.16056673648326014TruSeq Adapter, Index 1 (100% over 45bp)
CGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT530110.15402633396753834TruSeq Adapter, Index 1 (100% over 43bp)
CTTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC528660.15360502860779615TruSeq Adapter, Index 1 (100% over 42bp)
GGAAACTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA519240.15086799654657448TruSeq Adapter, Index 1 (100% over 40bp)
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAG494500.14367965544311126Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTCGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA491360.14276731142270405TruSeq Adapter, Index 1 (100% over 40bp)
AACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC491270.142741161434858TruSeq Adapter, Index 1 (100% over 44bp)
AGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC483260.14041381251655802TruSeq Adapter, Index 1 (100% over 44bp)
GCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCG463850.13477413180442296TruSeq Adapter, Index 1 (100% over 45bp)
CCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC457300.13287099380007034TruSeq Adapter, Index 1 (100% over 44bp)
CCGACCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT454310.1320022330927399TruSeq Adapter, Index 1 (100% over 41bp)
GACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC451390.13115381126484527TruSeq Adapter, Index 1 (100% over 44bp)
CGGACGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT446360.12969231749967064TruSeq Adapter, Index 1 (100% over 41bp)
ATTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT442350.12852719026341813TruSeq Adapter, Index 1 (100% over 43bp)
CTTCGGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA441370.1282424459513165TruSeq Adapter, Index 1 (100% over 40bp)
TCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT439850.1278008017121385TruSeq Adapter, Index 1 (100% over 43bp)
TCCGATATCGTTCAGCGGTTAGGATATAAGATCGGAAGAGCACACGTCTG430610.12511606962660898Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC428790.12458725876127741TruSeq Adapter, Index 1 (100% over 44bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCG415790.1208100382946233TruSeq Adapter, Index 1 (100% over 45bp)
CCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC411820.11965653327519125TruSeq Adapter, Index 1 (100% over 44bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCG411490.11956064998642235TruSeq Adapter, Index 1 (100% over 45bp)
TCGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT402830.1170444400447897TruSeq Adapter, Index 1 (100% over 43bp)
CTTCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC401910.11677712905791879TruSeq Adapter, Index 1 (100% over 42bp)
TCCGATACCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTG389000.11302605857911079Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT372980.1083713607425109TruSeq Adapter, Index 1 (100% over 43bp)
ATCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC367370.1067413448334394TruSeq Adapter, Index 1 (100% over 44bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAAGATCGGAAGAGCACACGTC365050.1060672562578519No Hit
CCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC364020.10576798417472469TruSeq Adapter, Index 1 (100% over 44bp)
TCCGATATCGTTCAGCGGTTAGGATATGGAGATCGGAAGAGCACACGTCT358730.10423094600021697Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGT356810.10367307959283424TruSeq Adapter, Index 1 (100% over 46bp)
GGAAACTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA356650.10362659072555233TruSeq Adapter, Index 1 (100% over 40bp)
CCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCG355170.1031965687031948TruSeq Adapter, Index 1 (100% over 45bp)
AAGTCTCTAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA350080.10171764161278946TruSeq Adapter, Index 1 (100% over 37bp)
TTTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT348810.10134863622873942Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)