FastQCFastQC Report
Wed 7 Aug 2019
HTLFWBGX7_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTLFWBGX7_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401626742
Sequences flagged as poor quality0
Sequence length99
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47869891.191899965665135No Hit
GTATATGTCTATGCCTTGCCCTTAAAGTAACTGACAATAAAAAGATTCTT4334780.10793056205405764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA1677800.029.3566235
CGCAGAG1675300.029.3046768
AACGCAG1938400.026.1103846
TCAACGC2173300.023.0901954
ACGCAGA2351550.021.2961757
ACTGTGC1136400.020.6377378
CTGTGCA946550.020.4768339
GCAGAGT2437350.020.2971329
CTATGCC2575050.019.2027289
ATCAACG2656100.018.8994223
CAGAGTG1643550.018.89202710-11
TCTATGC2392450.018.7335198
ACCTAGT824700.018.406891
CCTAGTA936800.018.2420332
AGAGTGA2112250.017.93410510-11
GAGTCCA869700.017.771299
CAGCGTG1228500.017.7668959
AGAGTCC959400.017.7224068
ACAGCGT1222150.017.6954638
TATACTG1990700.017.6580815
Produced by FastQC (version 0.11.5)