FastQCFastQC Report
Wed 7 Aug 2019
HTLFWBGX7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTLFWBGX7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401626742
Sequences flagged as poor quality0
Sequence length8
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACGCT19022052947.36251576594469No Hit
CGAGGCTG17492939243.555215255063864No Hit
GGGGGGGG237692075.918233153906868No Hit
CGCGGCTG15171060.3777402850331117No Hit
GCTAGCTA12408110.3089463101538194No Hit
GAGGCTGA4073200.10141754953159968No Hit
CCAACCAT4066910.10126093645427625No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)