FastQCFastQC Report
Wed 7 Aug 2019
HTLFWBGX7_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTLFWBGX7_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401626742
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCTAG80967700.020.932795
TTCTAGC80966500.020.9253066
TACTAGG56038400.020.9101896
GTACTAG56104900.020.8964425
TCTAGCA81279700.020.8951247
GATCCGT57830100.020.8948965
CTGTCCG59716900.020.8948577
TAGCATC81192150.020.8928729
TGTCCGC58027800.020.89258
CTAGCAT81245300.020.891858
TCTGTCC59833700.020.8869386
GATTCTA23250950.020.876834
TATTCTA19665750.020.8762444
CGTTATC61270000.020.8710469
GTCACTA55727450.020.8677838
ACTAGGT56382100.020.8663527
TTCTGTC59941400.020.8563255
GTCCGCA58091450.020.8548479
AGTCACT55804300.020.8520327
GTTATCC61263000.020.85196710
Produced by FastQC (version 0.11.5)