FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15871350
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGAGTACGATATATACTGAAACGAAAACGTTCTAATCTCTTGTTCC235380.1483049646060354No Hit
CGTACGTCTACTACTCTACTGTTCCTCTCGATATTCTTCCCTCATAGACT215340.1356784394522205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT666750.093.276211
AGTACGA1023300.091.801727
GAGTACG1025200.091.793756
TGTCGCG260150.090.362533
CGCGTCA253800.090.3585056
GCGCACT256950.090.304487
CGTCGTA204900.090.085641
CGTGTCG480700.089.881651
CGTATGT469600.089.780891
GTGTCGC266050.089.6976552
GTACGAT1046450.089.566588
GCGCGTC259800.089.533245
CGACTAT344450.089.47041
TCGCGCG264350.089.429973
CGCGCGT261300.089.346764
CGCGCAC260550.089.311986
GTATGAG1078100.088.8892442
CACGTCG307600.088.86974
CGAGATG219350.088.6986541
ATGAGTA1063900.088.669284