FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l02_n02_a35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l02_n02_a35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGTGCGTCTCGTCATCTCGAGATCGGAAGAGCGTCGTGTAGGGAAAG375.0Illumina Single End PCR Primer 1 (100% over 28bp)
CGTCGTGCGTCTCGTCATCTCGAGATCGGAAGAGCGTCGTGGAGGGAAAG125.0Illumina Single End PCR Primer 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)