FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l02_n02_a316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l02_n02_a316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTCGCGCGTGAGTATGTCGAGATCGGAAGAGCGTCGTGTAGGGAAAG1762.96296296296296Illumina Single End PCR Primer 1 (100% over 28bp)
CGTTTCGCGCGTGAGTATGTCGAGATCGGAAGAGCGTTGTGTAGGGAAAG13.7037037037037033Illumina Single End PCR Primer 1 (96% over 28bp)
CACGACGCGCGTGAGTATGTCGCAAGGGTGTTTTTTATCATTAAATAAGC13.7037037037037033No Hit
CGACACGCGCGTGAGTATGTCGACGCGTGATCAGTAGAAACAAGGGTATT13.7037037037037033No Hit
CGTGTCGCGCGTGAGTATGTCGACGCGTGATCAGTAGAAACAAGGGTGTT13.7037037037037033No Hit
CGTGGCGCGCGTGAGTATGTCGAGATCGGAAGAGCGTCGTGTAGGGAAAG13.7037037037037033Illumina Single End PCR Primer 1 (100% over 28bp)
CGTGTCGCGCGCGAGTATGTCAAGATCGGAAGAGCGTCGCGTAGGGGAAG13.7037037037037033Illumina Single End PCR Primer 1 (95% over 24bp)
CGTGTCGCGCGTGAGTATGTCGAGATCGGAAGAGCGTCTTGTAGGGAAAG13.7037037037037033Illumina Single End PCR Primer 1 (96% over 28bp)
CGTGTCGCGCGCGAGTATGTCGAGATCAGAAGAGCGTCGTGTAGGGAAAG13.7037037037037033Illumina Single End PCR Primer 1 (96% over 28bp)
CGTGTGGCGCGTGAGTATGTCGAGATCGGAAGAGCGTCGTGTAGGGAAAG13.7037037037037033Illumina Single End PCR Primer 1 (100% over 28bp)
CGTGTCGCGCGTGAGTATGTCGAGGGGCCGTGTCGCGCGTGAGTATGTCG13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)