FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15871350
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGTCTACTACTCTACTGTTCCTCTCGATATTCTTCCCTCATAGACT220140.13870275685433187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT682800.093.7518161
AGTACGA1051600.092.207147
CGTCGTA186550.092.153611
GAGTACG1055000.091.990456
CGACTAT319350.091.459281
GTACGAT1072200.090.343128
CGTGTCG433850.090.278021
CGCGCGT249950.090.130834
CGTATGT470950.089.8684161
CGCGTCA250250.089.854226
GCGCGTC250750.089.826595
TCGCGCG252700.089.54473
TGTCGCG247700.089.511373
CGAGATG196800.089.289221
GCGCACT246800.089.2439967
CGCGTCT63100.089.180161
GTATGAG1098100.089.130252
GTGTCGC249950.089.0666662
TGAGTAC1091200.088.999665
GTGTATA718550.088.9141542