FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l02_n01_a292.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l02_n01_a292.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATGAGTCTATATACGTCGAGATCGGAAGAGCACACGTCTGAACTCC350.0Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAGATGAGTCTATATACGTCGAGAGCGGAAGAGCACACGTCTGAACTCC233.33333333333333Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CGAGATGAGTCTATATACGTCGGGATCGGAAGAGCACACGTCTGAACTCC116.666666666666664Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)