FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12557126
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGAGTACGATATATACTGAAACGAAAACGTTCTAATCTCTTGTTCC234680.1868898982139703No Hit
CGTACGTCTACTACTCTACTGTTCCTCTCGATATTCTTCCCTCATAGACT211010.16804004355773766No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT150550.11989208358664236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT657050.093.655331
GAGTACG1005300.092.0941246
AGTACGA1005150.092.0558857
CGTATAG638650.091.809261
GCGCGTC238600.091.675375
CGCGTCA236700.090.9864656
TGTCGCG244100.090.894083
TCGCGCG244500.090.842523
GTGTCGC248000.090.7479552
CGACTAT336700.090.73951
TCACGTC292600.090.6330953
CGCGCGT241200.090.628084
CACGTCG290250.090.532274
CGTGTCG415650.090.3031161
GCGCACT243050.090.250957
CGCGCAC243550.090.182696
GTACGAT1024500.090.141028
ATGAGTA1031550.090.1051564
CGTCGTA174700.089.915561
GTATGAG1052300.089.804452