FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156034
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTG3770.24161400720355822No Hit
GGGCATTTTGGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACG2760.17688452516759168No Hit
CGAGCTCTATACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACC2750.17624363920683953No Hit
CGAGTATACGTACGTCTCAGTCCAGGAGGGAAGAATATCAACAGGAACAG2520.16150326210954022No Hit
CGAGTATACGTACGTCTCAGTCCGGGGGAGAATATCAACAGGAACAGCAG2450.1570170603842752No Hit
CGAGTATACGTACGTCTCAGTCCGGGGAAGAATATCAACAGGAACAGCAG2400.1538126305805145No Hit
GGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTG2170.1390722534832152No Hit
CTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACAAATCCTAAAA1910.12240921850365945No Hit
CGAGTATACGTACGTCTCAGTCGAGGGGAGAATATCAACAGGAACAGCAG1690.1083097273671123No Hit
CGAGTATACGTACGTCTCAGTCAGGGGAAGAATATCAACAGGAACAGCAG1640.10510529756335159No Hit
TGGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGT1600.10254175372034299No Hit
CGAGTATACGTACGTCTCAGTCAAGGAGGGAAGAATATCAACAGGAACAG1570.10061909583808656No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATAC156.6071155E-495.07
TCGTGTG301.2249075E-895.08
CGCTATC156.6071155E-495.01
GTATCTA301.2249075E-895.05
CGAGCTT156.6071155E-495.01
TGACGTA254.601843E-794.999994
TACGCAC254.601843E-794.999997
ACTACGA409.094947E-1294.999995
TCGTACT201.7390881E-594.999997
ATACGCA201.7390881E-594.999996
CGACTAG254.601843E-794.999999
ACTCACG201.7390881E-594.999997
CGTCGTA3550.088.309861
GTGTCGT650.087.69232
CGACATA600.087.0833361
CGACTGC500.085.499999
CGAGCTC15200.084.375011
GTACGCT3750.083.65
CGAGTAT66650.083.454611
GCTGTCG3650.083.2876749
Produced by FastQC (version 0.11.5)