FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44958
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC3330.7406913118910984No Hit
CGACGACGACACCACGCCCCCTCAGATTGCCTGATGGGCCTCTTTGCCAT810.1801681569464834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG254.5483284E-795.03
GTGTCGC156.576271E-495.02
ACGCTGT254.5483284E-795.07
TAGTGTA156.576271E-495.07
AGCTACG156.576271E-495.05
TCGCGCG254.5483284E-795.03
GCGCGTC254.5483284E-795.05
CGTAGAG301.2050805E-895.01
CGCTAGA450.095.01
CGCTACA254.5483284E-795.01
GCTACGT156.576271E-495.06
GTGAGCT156.576271E-495.02
GCTACAC254.5483284E-795.02
GCTATAC156.576271E-495.02
ATAGTCG450.095.03
ATACGTA156.576271E-495.06
AGAGTAC156.576271E-495.03
TCTCGCA156.576271E-495.06
AGTCGTA450.095.05
GAGTACA156.576271E-495.04
Produced by FastQC (version 0.11.5)