FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92335
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGAGCTACGTCAATGCAGCAGCCTACGGGGAAATGAAGCAGGGGGCTG2350.2545080413710944No Hit
CGTGAGCTACGGGAACAGATTGATAACGTTTACTAAACAGACCCAGACTT1730.18736123896680565No Hit
CGTGAGCTACGCTGTGTGTGTGCATGTGCGGGTGTGTGAGAGTGTGTGTG1540.1667839930687172No Hit
CCCATCCTGAAGCAGGTCACATCAGCCTCAAACGTCATCCAGAGGACCAC1240.13429360480857747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGT450.095.000019
GTAGCTA450.095.000012
TATAGTA201.7353072E-595.000017
CGACTAT201.7353072E-595.000011
ACTCACG201.7353072E-595.000017
TATGTAT450.095.000013
GTCGCGT254.5868364E-795.06
TAGACGA500.095.05
GAGTACA1050.095.04
CGACATA500.095.01
CTAGTCG254.5868364E-795.03
TCGTACT254.5868364E-795.07
CGTGTCG550.095.01
CGAGATG550.095.01
CGAGAGT1100.095.01
CGATAGT254.5868364E-795.01
TCGCGTG254.5868364E-795.07
TAGTCGC254.5868364E-795.04
ACACGCG156.598492E-494.999995
GTCGATA156.598492E-494.999992
Produced by FastQC (version 0.11.5)