FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90546
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC5500.6074260596823714No Hit
CGACGAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAACGCG2380.26284982218982617No Hit
ACGCGTGATCAGTAGAAACAAGGTATTTTTTCATGAAGGACAAGTTAAAT1420.15682636449981224No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT1180.13032050007730878No Hit
CGACGAGTCACCCAGGGGAGTGGAGGAAGGCTCTATTGGGAAAGTGTGCA1170.12921608905970444No Hit
CAGGAAAGAACACAAATCCTAAAATTCCCTTAGTCAGAGGTGACAAGATT1130.1247984449892872No Hit
CACCATGGACACAGTGGTGGAGGCCATGGTGTCTAGGGCCCGGATTGATG1060.11706756786605704No Hit
CAGCAAATATCATTGGGATCTTGCACCTGATATTGTGGATTACTGATCGT1060.11706756786605704No Hit
CGACGAGTCTCTGTGTTTGTCGTCCACTTTCCCTTTTCTGAGTATTGGTG1000.11044110176043118No Hit
ACGCGTGATCAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAAT950.1049190466724096No Hit
GGGCATTTTGGATAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACG910.10050140260199236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAG450.095.02
CGAGTCC156.59807E-495.04
CGAGTAG450.095.01
TAGACGA650.095.05
GAGTACG156.59807E-495.06
TGAGTAC156.59807E-495.05
CGCTGAC156.59807E-495.01
GTCAGTC600.095.02
AGTATAC500.095.03
ACGATGT254.5861088E-795.05
ACGATCG450.095.08
TCGTGTG156.59807E-495.08
TCTACTA254.5861088E-795.07
ATGAGTA156.59807E-495.04
CTAGTCG156.59807E-495.03
GTATGTA301.2190867E-895.02
ACAGCTA450.095.05
GTACTCA500.095.09
CATACGA450.095.08
CGAGCTC254.5861088E-795.01
Produced by FastQC (version 0.11.5)