FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8018
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGAGTGCGGGACTGCGACATTAGATCTCTCAGTTCTTTTATTCTCTCC871.0850586181092543No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC150.18707907208780244No Hit
ACGCGTGATCAGTAGAAACAAGGCGATCTTCCAGGAGCTAATGGTAGTGC140.1746071339486156No Hit
CGCGAGCGAGCATGCGTGTACACACACGTGTGCATGTGGCAGGGCAGCAG130.16213519580942878No Hit
CGCGAGCGATTCAAGTGATCCTCTCGTCATTGCAGCAAATATCATTGGGA110.13719131953105512No Hit
CGCGAGCAAGCCACATCTCGGGACCCCTTCTTGGAAAACTCGCAAACCCA110.13719131953105512No Hit
ACGCGTGATCAGTAGAAACAAGGCTATGGAAGCTTCAGATCCTGGGAGGG110.13719131953105512No Hit
CGCGAGCGAGAGCAATTGGGACAGAAATTTGAGGAAATAAGGTGGTTAAT100.1247193813918683No Hit
CGACAACCAACTTCAATTCTACCAGCAGATAAAAAGCCTGATCAAGGTAA90.11224744325268148No Hit
CGCGGGTGCGGGACTGCGACATTAGATCTCTCAGTTCTTTTATTCTCTCC90.11224744325268148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACTA201.6396923E-595.000019
GTACACA201.6396923E-595.000016
TACACAC201.6396923E-595.000017
AGTACAC201.6396923E-595.000015
AGAGTAC201.6396923E-595.000013
GAGTACA201.6396923E-595.000014
CTACGAC201.6396923E-595.000016
GAGAGTA201.6396923E-595.000012
CGAGAGT201.6396923E-595.000011
CGAGACG201.6396923E-595.000011
AGACTAC201.6396923E-595.000013
CGACTGC201.6396923E-595.000019
GCGAGCG201.6396923E-595.000012
ACTACGA201.6396923E-595.000015
GTATGTA156.378235E-495.02
GCGGGAC254.212343E-795.08
CGTCAGT156.378235E-495.01
GTCGCGA156.378235E-495.07
TATGTAT156.378235E-495.03
ACGTAGA156.378235E-495.05
Produced by FastQC (version 0.11.5)