FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100024
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGAGTACACACTACTAACCATTAATCTTGCGTAGCAGAGGCTCGAGTT2850.2849316164120611No Hit
CGAGAGTACACACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAAT2110.21094937215068382No Hit
CGAGAGTACACACACACAATACACACATATAAACGTATTCTTATATGTTC1840.183955850595857No Hit
CGAGAGTACACTACTAACCATTAATCTTGCGTAGCAGAGGCTCGAGTTTT1400.13996640806206512No Hit
ACGCGTGATCAGCAAAAGCAGGTTGATGTGCTAAATGTCAATTATTTCAA1130.11297288650723826No Hit
ACGCGTGATCAGTAGAAACAAGGCGATCTTCCAGGAGCTAATGGTAGTGC1120.1119731264496521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACG254.589674E-795.03
GAGTACT254.589674E-795.04
CGCATAG156.600117E-495.01
CGCGCGT301.22036E-895.04
CGTCTCG600.095.06
CGACATA550.095.01
CGACAGC156.600117E-495.04
CGCTCAT254.589674E-795.01
CTAGTCG254.589674E-795.03
CGCTATC301.22036E-895.01
CGTACTC156.600117E-495.08
CGTGTAT156.600117E-495.01
CGAGAAT301.22036E-895.01
GCGTGAC254.589674E-795.02
CACGTCG254.589674E-795.04
CGCGTCA301.22036E-895.06
GCGTCAC301.22036E-895.07
GAGGGTA156.600117E-495.02
CGGAGTA650.095.01
AGTACCC156.600117E-495.05
Produced by FastQC (version 0.11.5)