FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36105
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG201.7209459E-595.000011
TAGACGA201.7209459E-595.000015
CATACGA201.7209459E-595.000018
CGTGTCG450.095.000011
CGAGAGT450.095.000011
TCATACG201.7209459E-595.000017
AGTAGAC201.7209459E-595.000013
ATACGAC201.7209459E-595.000019
CGATCGA201.7209459E-595.000019
CGAGTAT301.1983502E-895.01
CGTGAGC500.095.01
CGCATAG156.565669E-495.01
GTCTATC254.5300112E-795.02
CGTCTAT254.5300112E-795.01
GTGTCGC156.565669E-495.02
TAGTGAT156.565669E-495.07
CGTCATA254.5300112E-795.01
TGTCGCG156.565669E-495.03
CGAGATG353.1832315E-1094.999991
CGACTGC353.1832315E-1094.999999
Produced by FastQC (version 0.11.5)