FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130708
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGACTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACA2970.2272240413746672No Hit
CGAGACTACTGCAAGCCCATACACACAAGCAGGCAGGCAGGATTTATGTG2770.21192275912721484No Hit
CGAGACTACGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGA2350.17979006640756495No Hit
CGAGACTACGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCG2100.16066346359824954No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC1630.12470545031673655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT409.094947E-1295.000011
GCTACGT201.738027E-595.000016
TAGTGTA201.738027E-595.000017
GTGACGT201.738027E-595.000014
ACTCACG409.094947E-1295.000017
CGAACTA750.095.01
GCTATCA156.604696E-495.02
CGCGCAC500.095.06
CGTCTCG1850.095.06
TACGGCT156.604696E-495.07
CTGACGT254.597623E-795.03
GCTGACG254.597623E-795.02
GCGCACT500.095.07
ACGATGT750.095.05
CGCTCAT301.2234523E-895.01
TACGATG156.604696E-495.07
CGCTATC156.604696E-495.01
GTGTCGT1850.095.02
GTCGTCT1800.095.04
CATACGA1800.095.08
Produced by FastQC (version 0.11.5)