FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173593
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTCGTTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCAC9620.554169810994683No Hit
CGTGTCGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATA7800.4493268737794727No Hit
CGTGTCGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTGTTT5350.3081921506051511No Hit
CGTGTCGTCTGACAAGTTGTATTGCAATCGTGGACTGGTGTATCTGAAAT4530.2609552228488476No Hit
CGTGTCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCGAGAAAATTGC4430.2551946219029569No Hit
CGTGTCGTCCATCATAATCACTGAGTTTGAGTTCAGTGCACATTTGGATG3020.1739701485658984No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC2530.14574320393103407No Hit
CGTGTCGTCTGAATTTGTGTGTCTCCCCTATGGCACCTATATGTGTATCT2460.14171078326891062No Hit
CGTGTCGTCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA1770.10196263674226497No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGT201.7396424E-595.000016
ATCTATA409.094947E-1295.000017
CTATAGT409.094947E-1295.000019
GTCTCTC409.094947E-1295.000017
TATCTAT409.094947E-1295.000016
GTATCTA409.094947E-1295.000015
CTCGCAT201.7396424E-595.000017
GTCCCGA201.7396424E-595.000017
AGCTACT409.094947E-1295.000017
TAGTCGC201.7396424E-595.000014
TCTATAG409.094947E-1295.000018
CGTATCG156.6083804E-495.01
CGTATAG353.274181E-1095.01
CTGACGT700.095.03
GCTGACG700.095.02
CGCTCAT254.604044E-795.01
CATACGA1900.095.08
ACGTCAC450.095.08
CGAGATG1700.095.01
GCGTGAC301.225817E-895.02
Produced by FastQC (version 0.11.5)