FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n02_44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n02_44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138858
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGACATCCGACATGCGAACAGAGGTTATAAGAATGATGGAAAGTG3410.24557461579455273No Hit
CGAGTAGACGATCTCGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAA3380.24341413530369155No Hit
CGAGTAGACAATCCACGCCCTAATGATAAGACAGGCAGTTGTGGTCCAGT2730.19660372466836626No Hit
CGAGTAGACGAGCAGTCAATTGCTGAGGCAATAATTGTGGCCATGGTATT2500.1800400409050973No Hit
CGAGTAGACTTTGATGACTGCAAAGATGTTGGAGACCTTAAACAGTATGA2230.16059571648734677No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC1980.14259171239683704No Hit
CGAGTAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCA1760.1267481887971885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA500.095.000012
CTATCAC254.599151E-795.000013
GCTATCA254.599151E-795.000012
TGTGATA254.599151E-795.000015
AGTGACG201.7384107E-595.000012
GCTACGT201.7384107E-595.000016
CGACTAT201.7384107E-595.000011
AGCTACG201.7384107E-595.000015
GTGACGA201.7384107E-595.000013
CGATACG254.599151E-795.000011
TCGCGCG500.095.000013
GACTATA201.7384107E-595.000012
ATACACG201.7384107E-595.000018
TCGCATA156.605572E-495.08
AGATCGT301.223998E-895.04
GAGTATA600.095.02
ACGATCC156.605572E-495.08
CATAGTC156.605572E-495.03
CTAGTCG301.223998E-895.03
CGCTACA600.095.01
Produced by FastQC (version 0.11.5)